Asian Journal of Bio Science

M. Venkateshwarlu ¹

Affiliations
1 Department of Botany, Kakatiya University, Warangal (A.P.), IN

Abstract

Impact of various growth regulators on tissue and isolated plant cells was studied by several workers and these substances were known to play an important role in cell metabolism, cell membrane synthesis (Cocking, 1978) besides growth and differentiation (Butenko, 1968; Stress, 1969), of these growth substances auxins have proved to be an essential supplement for establishing successful culture of plant tissues (Gautheret, 1959; Rao and Swamy, 1972). Auxins were found to exert their hormonal action through their effects on the nucleic acids and protein metabolism. It was also found that auxins exert their effects, directly or indirectly on ‘S’ phase of DNA synthesis (Peaud-Lenoel, 1977). Cytokinins were found to influence cell growth by promoting nucleic acid metabolism and synthesis of specific proteins required for cell division. It was further reported that cytokinins bind to a specific receptor site in the cell to bring about organogenesis (Yamada et al., 1972). Physical environment viz., state of medium, light temperature humidity etc., besides source and size of explants are known to play an important role in the in vitro organogenesis.

Keywords

Leaf Explant, Morphogenetic Response, Cucumis melo cv. BATHASA and Growth Regulators.

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M. Venkateshwarlu ¹

Affiliations
1 Department of Botany, Kakatiya University, Warangal (A.P.), IN

Abstract

Conventional way of cloning is applicable to Zizyphus mauritiana species with 65% ischolar_maining branch cuttings treated with auxins. When rapid and large scale cloning of selected germplasm considered, the method was found unsuitable as it imposed extensive damage to valuable elite stock. Other drawbacks such as time consuming, slow and seasonal influences on ischolar_maining, etc. also limited extensive application of this technique, (Bajaj, 1986; Tiwari, 1992; Harry and Thorpe, 1994). Selection and rapid multiplication of ‘elite’ tree using micropropagation can produce stands which produce higher yields. Further selected varieties/strains which produce uniform, heavy crops of large sized seeds with maximum oil and alkaloid can be cloned to increase yield.

Keywords

Mliitipurpnse, Mocropropagation, Productivity, Zizyphus mauritiana.

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M. Venkateshwarlu ¹

Affiliations
1 Department of Botany, Kakatiya University, Warangal (A.P.), IN

Abstract

Cadmium was applied in the form of cadmium – chloride. Various growth regulators on tissue and isolated plant cells were studied by several workers and these substances were known to play an important role in cell metabolism, cell membrane synthesis besides growth and differentiation of these growth substances auxins have proved to be an essential suppliement for establishing successful culture of plant tissues. An attempt has been made to asses the response of Cucumis melo. It is evident from the results obtained that the increase in cadmium concentration affected all the growth parameters. The morphological parameters such as ischolar_main length and shoot length showed a decreasing trend with increase of cadmium chloride concentration.

Keywords

Cell Membrane Synthesis, Cadmium, Plant Tissues.

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M. Venkateshwarlu ¹, K. Sujatha ², Ch. Sridhar Rao ¹

Affiliations
1 Department of Botany, Kakatiya University, Warangal (A.P.), IN
2 2Department of Zoology (Sericulture Unit), Kakatiya University, Warangal (A.P.), IN

Abstract

One third of India’s population is dependent on wood fuel for cooking their daily meals, which generates tremendous pressure on the scanty vegetation. Although we have modern technologies and fast developing industrial sector, gas and electricity are neither available nor affordable for this large section of the population. The predominant Zizyphus tree species can survive well in high temperature, slight frost and low rainfall. Their ischolar_mains penetrate deeply in to ground water level and so they do not compete for water with the crop plants (Leaky and Last, 1980). Most of species are scattered widely throughout tropical and subtropical arid regions. Several of these are categorized as “multipurpose trees” and are backbone of rural economy throughout the drier plants of the world. It is because of the dependence on these species that plants have become over exploited. Tremendous pressure exerted by both man and animal, resulted in complete removal of superior germplasm or in some cases plant species have become threatened (Ramawat and Nadwani, 1991). The situation has become compounded by various inherent biological problems.

Keywords

Limitations, Prerequisite, Somaclonal Variation, Protoplast Culture, Zizyphus.

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